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KMID : 0644120140270010017
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Journal of Oriental Gynecology
2014 Volume.27 No. 1 p.17 ~ p.27
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Osteoclast Differentiation of Polygoni Cuspidati Radix Extracts Effects and Mechanism of Inhibition Studies
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Jang Hee-Jae
Hwang Deok-Sang
Lee Jin-Moo
Lee Chang-Hoon
Lee Kyung-Sub
Jang Jun-Bock
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Abstract
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Objectives: This study was conducted to evaluate the inhibitory effect of polygoni cuspidati radix (PCR) extract on osteoclast differentiation.
Methods: MTT-assay was performed to estimate cytotoxicity of PCR extract in BMMs stimulated with RANKL. Tartrate resistant acid phosphatase (TRAP) staining, TRAP activity and RT-PCR were performed to know the inhibitory effect on osteoclast differentiation. actin ring formation were analysed to observe the effect of PCR extract.
Results: PCR decreased the number of TRAP positive cells and TRAP activities in BMMs stimulated with RANKL and M-CSF. PCR restrained the formation of actin ring. PCR down regulated the induction of NFATc1, c-Fos, TRAP and OSCAR by RANKL. PCR inhibited NF-κB activity by inducing degradation of IκBα.
Conclusions: We suggest that PCR Extracts can be an effective therapeutic agent on osteoclast differentiation caused by diseases such as osteoporosis.
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KEYWORD
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Polygoni Cuspidati Radix Extracts (PCR Extracts), Osteoclast Differentiation, Tartrate Resistant Acid Phosphatase (TRAP) Activity, NF-κB, C-FOS, NFATc1
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